Technical Specifications. 1. Molecular aspects of color variation in luciferase emission have been the subject of numerous studies: various mechanisms have been proposed [6] and multicolor reporter proteins have been created and applied in cell-based. ®SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. ® The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Molecular Devices Corporation. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The VersaMax reader uses the best plate for your assay. SoftMax® Pro Software Validation Package provides the most comprehensive documentation and tools available to validate GxP administrator features, software. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. ®ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. The cortisol-driven molecular programming of HPI axis development has focused only on GR signalling in fishes 12,13,14,15,16,17. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Find regional support. SpectraMax Plus384, 190, 340PC384, and VersaMax Installation Guide Author: Molecular Devices Subject: Installation Guide Keywords: SpectraMax Versa and others Detection Platform Created Date: 5/17/2023 2:14:27 PMThis version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. 4. The optical density (OD) of the mixture was measured at 490 nm with a microplate absorbance reader (VersaMax (Molecular Devices), Shanghai, China). 3 cells (6x105) were ocExtracts were placed in a 96-well plate in triplets and spectrophotometric readings were taken at 555 nm on a microplate reader (Versamax, Molecular Devices LLC, USA). To evaluate the reliability of the assay, SkinEthic TM RHE (1% Triton X-100) was used. The primers for the human genes GAPDH, SOD1, SOD2, CAT, GPX1, CCS, PRDX6, FOXO3, CDKN2A,. +81-3-5282-5261 SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. StakMax Microplate Handling Stacker. VERSAmaxTM Tunable Microplate Reader Operator s Manual for Instruments with Serial Numbers Beginning. 8. Lihat Video. , Sunnyvale, CA). The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both. Absorbance was read at 412 nm every minute for 10 min using a VersaMax (Molecular Devices, San Jose, CA) spectrophotometer. The amount of MTT reduction was measured immediately by detecting absorbance in a microplate reader (Versamax, Molecular Devices) at a wavelength of 570 nm and a reference wavelength. This. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. And the VERSAmax reader is robotics-compatible. The rate of cell viability was calculated using the following formula:The conversion of NADP+ to NADPH was measured as absorbance (340 nm) on a microplate reader (VersaMax, Molecular Devices Co. 00. VERSAMAX. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. NORTH AMERICA. Molecular Devices Versamax Tunable Microplate Reader is available from The Lab World Group. The plates were incubated at RT for an additional 25 min to stabilize the luminescence signals followed by the measurement of luminescence using a microplate reader (VersaMax, Molecular Devices, CA, USA) (n = 6). The limit of detection for each assay was 156. Used. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 47 shipping. VersaMax ELISA Microplate Reader. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Formerly known as MicroMax, Molecular Devices’ unique SpectraDrop™ Micro-Volume Microplate, offers the highest throughput solution for low volume measurement available onthe market today. For validation of the Molecular Devices VersaMax™ Absorbance Microplate Reader for use with the Pyrochrome Assay, data acquisition and analysis were performed with SoftMax ® Pro GxP Software. The absorbance was measured at 450 nm with 650 nm as a reference on a VersaMax (Molecular Devices, Sunnyvale, CA) and a standard curve was plotted using a 4-parametric mathematical fit model in. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. +49-89-9620-2340 Fax +49-89-9620-2345 Japan Nihon Molecular Devices Tel. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard. Invasion assayCells were seeded into the top chamber of a 96-well matrigel-coated plate with 8-m-pore polyethylene terephthalate membrane inserts (Corning). . 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). Absorbance was read at 412 nm every minute for 10 min using a VersaMax (Molecular Devices, San Jose, CA) spectrophotometer. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. Table of Contents. 0 out of 5 $ 2,999. Wavelength Range. 5 mM EDTA, 0. SpectraMax® i3 Multi-Mode Detection Platform. The average survival rates for cells exposed to dry. used. The sections were stained with hematoxylin and eosin (H&E) and. 8. Published Dec 16, 2020 | Updated May 19, 2023. This manual does not represent a commitment on the part of Molecular Devices Corporation. Windows Home Editions are not supported. (VersaMax, Molecular Devices, Sunnyvale, CA, US). Turn the reader on and secure the USB cable. HT22 cells are derived from hippocampal neuronal HT4 cells. Study conductThe amounts of chlorophyll a and chlorophyll b were measured at 664. Patients with small intestinal neuroendocrine tumors (SI-NETs) frequently develop spread disease; however, the underlying molecular mechanisms of disease progression are not known and effective. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. Although this manual has been prepared with every precaution to ensure accuracy, Molecular DevicesSpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing. Spectranet customer portalSpectraMax and VersaMax . An aliquot of the samples dissolved in DMSO with 80 μL of phosphate buffer (pH 6. (Seongnam, Korea). Proliferation of the released daughter cells was monitored at a wavelength of 578 nm online by a microplate reader (VersaMax; Molecular Devices, Sunnyvale, Calif. To update the driver. ). Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. 635. With optimized reagents, option to operate the. With its advanced features and user-friendly interface, the VersaMax is the perfect choice for a wide range of research. reader (VersaMax, Molecular Devices, Sunnyvale, CA, USA) at 560 nm with background subtraction at 630 nm, based on which the corresponding cell viability was assessed. Flexible calculations Data analysis features are available such as 21 different curve fit options, simplified cross-plate analysis and interpolation, and automatic calculations. Wavelength Selection. com we know that an user's manual may be needed at any time. 003 OD ±1. 00 Add to cart Molecular Devices Microplate Reader Spectramax 384 340PC $ 6,295. To provide access to disconnect power from the instrument, maintain a 20 cm to 30 cm (7. , Sunnyvale, CA). To make your request, nothing more simple: you just have to connect on the site and to make your request on line. This manual is subject to change without notice. We would like to show you a description here but the site won’t allow us. The samples were incubated with FBS, and the optical density of particles was determined with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA) by measuring the absorbance at 560 nm for 2 h. We would like to show you a description here but the site won’t allow us. A. SpectraMax®, VersaMax, VMax. Briefly, MTT (200 μl, 2 mg/ml) was added to the wells and incubated for 4 h at 37°C. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. After freeze-drying, the. 0 nm full width half maximum. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. After treatment, cells were collected, lysed with the lysis buffer. Used. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. Glossary of Terms. Select up to two wavelengths. Absor-bance of the solution was measured using a micro plate Reader (VersaMax, Molecular Devices) at 570 nm. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 9 in. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The SpectraTest® ABS1 Absorbance. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Molecular Devices E Max Precision Microplate Reader. The signal was read on an ELISA plate reader (SpectraMax VersaMax, Molecular Devices, Sunnyvale, CA, USA). VersaMax ELISA Microplate Reader. The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). $1,195. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and. 72 MB. 파장 정확도 : < ±0. spectrophotometer (VERSAmax, Molecular Devices). The preparation of cytoplasmic and nuclear proteins was performed by using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Invitrogen, USA) according to. P. from Molecular Devices Corporation. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. We would like to show you a description here but the site won’t allow us. Molecular Devices VersaMax Microplate Reader Molecular Devices VersaMax Microplate Reader Specifications. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer. 0A Sunnyvale, CA Made in USAVersaMax price. Furnace Manuals. The index was evaluated at 48 and 72 hours normalized to that at 24 hours. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. To evaluate the reliability of the assay, SkinEthic TM RHE (1% Triton X-100) was used. ) gap between the rear of the instrument and the wall. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. This technical note is designed to provide information . The VersaMax measures thMolecular Devices memberdayakan para ilmuwan untuk memajukan diagnosis dini dan terapi yang lebih aman untuk pasien. Table 1. 0 OD Kinetic Read Time: 9 Second Minimum Interval Temperature Uniformity ±0. The absorbance was measured at 405 nm using a plate reader (VersaMax, Molecular Devices) (N = 3). The absorbance was measured using a microplate reader (VERSAmax; Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 530–560 nm. After incubation for 1 h, the medium was transferred to 96-well plates (SPL Life Sciences) in triplication and absorbance was measured by using the ELISA microplate reader (VersaMax, Molecular Devices, USA) at 490 nm. 제조사 : Molecular Devices. The absorbance was assumed to be 100% for the control group. The system provides automation for up to 50 For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The assay procedure was in accordance with the manufacturer's instructions, and the results were analyzed with a microplate reader (VersaMax; Molecular Devices Ltd. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. versamax, vmax, emax, uvmax, flexstation, softmax pro 5 gxp software, spectratest, molecular devices automation partners, gemini, lmax, assay protocols, data display, data analysis, assay. Substrates and coenzymes provided and specified in the kit were added at 37°C after 1 min plate was read in a spectrophotometer (Versamax, Molecular Devices, San Jose, CA, USA) at 37°C every 1 min for 4 min. The VersaMaxTM Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Then, the cultured cells were treated with different. All analyses were performed three times, and the mean values were used for further calculations. Manufacturer: Molecular Devices Model: VERSA MAX Turnable Microplate reader Further Information Descriptions, data, dimensions and quantities are based on the subjective beliefs of the auctioneer and are created to the best of our knowledge and belief. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. g. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. SpectraMax and VersaMax Plate Readers Operator s Manual -- 0112-0126 Rev A Molecular De. 0A-Sunnyvale, CA-Made in USASpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Youmustensurethatthemeniscusiscomfortablyabove thelightbeaminstandardcuvettesandthatthesamplechamberinamicrocuvetteis Microplate Reader Capability > UV - Vis Absorbance. Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. Molecular Devices memberdayakan para ilmuwan untuk memajukan diagnosis dini dan terapi yang lebih aman untuk pasien. Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. VERSAMAX. p-nitrophenol (Sigma) with diluted concentrations ranging from 0 to 0. Molecular Devices versamax Operator's Manual (47 pages) Tunable Microplate Reader. The samples were incubated with FBS, and the optical density of particles was determined with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA) by measuring the absorbance at 560 nm for 2 h. The growth inhibitory rate (GIR) of treated cells was calculated by Equation 1. 747. The absorbance was measured at 450 nm using a microplate reader (VersaMax, Molecular Devices). The absorbance at wavelength 450 nm was detected using a micro-plate reader (VersaMax, Molecular Devices, USA). SoftMax® Pro, the industry-leading microplate reader software, is included at no additional charge. Monday to Friday 8am - 5pm GMT. For more sample throughput, the system can be easily integrated into a full robotic The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Cell viability was represented by percentage values compared to a control and then used to calculate the number of viable cells. All cell lines were cultivated for 24 h in 96-well cell culture plates with an initial concentration of 1×10 5 cells/mL. Multichannel optics. SpectraMax® Microplate Reader User Manual Download Page. A series of 44 excipients were evaluated at six different concentrations in triplicate. #VERSAMAX) SoftMax Pro GxP Software version 5. No accessories are needed and SoftMax® Pro Software. 5 × 10 6 cells per well inoculated into 6-well plates in culture medium with CSF1 (10 ng/mL) for 7. The mouse hippocampal neuronal cell line HT22 is frequently used as an in vitro model to investigate the role of hippocampal cholinergic neurons in cognitive functions. SoftMax Pro. Select Update Driver. Plates values were acquired using a fluorescence microplate reader (570 nm; Versamax, Molecular Devices, San Jose, CA, United States). Monday to Friday 7am - 5pm Pacific Time. 5×10 3 cells were seeded in 96-well plates, treated with indicated inhibitors for 48 or 72 hours, analyzed 16 hours after BrdU addition, and absorbance measured at 450nm using a spectrophotometer plate reader (VersaMax, Molecular. Microplate assays involved a 96-microplate reader (VERSAmax; Molecular devices, Sunnydale, California, USA) used with flat-bottomed 96-well microplates (NUNC, Sigma Aldrich, UK). Ann Arbor, MI, USA). The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. The wells were visualized using o-phenylenediamine (OPD), and the absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 490 nm. S. For the MTS assay, the CellTiter 96® AQ ueous One Solution Cell Proliferation Assay kit was used following the manufacturer's instruction. Measurement of biofilm formation inhibitory activity. The optical density was measured at a wavelength of 450 nm using VersaMax (Molecular Devices, Seoul, South Korea). The optical density of each well at 490 nm was then measured using a microplate spectrophotometer system (VersaMax; Molecular Devices, Sunnyvale, CA, USA). The. 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All. The collaborative space brings customers and researchers into the lab to test automated workflows for organoid culturing and screening, with guidance from in-house. 0 software (GraphPad). 00 USD Tecan Infinite F200 Reader Fluorescence Reader w WARRANTY $2,750. Direct cytotoxicity of HVJ-E and Laserphyrin®-HVJ-EThe MTT formazan crystals were dissolved by adding 50 μl DMSO to each well and the plates gently shaken until the MTT solution was dissolved. Our specialization servicing microplate instrumentation and our focus on a narrow range of product lines allows us to provide the highest standards of service and ensure. Briefly, 100 μl of cell culture medium was mixed with 100 μl of Griess reagent [equal volumes of 1% (w/v) sulfanilamide in 5% (v/v) phosphoric acid and 0. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0140 Rev. Spectrophotometers. TECHNICAL NOTE. Table of Contents. The emodin thermoreversible PG was. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Order Now. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. Windows 10 – 2004/20H2/21H1/21H2. 1. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The absorbance value (OD) was measured at 492 nm with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA). SoftMax Pro 7. Absorbance was read on a spectrophotometer (VersaMax, Molecular Devices) with SoftMax Pro GxP v5 software. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. JEG-3 cells were transfected with myc-tagged NDRG1 wild type or deletion mutants at the N- or C- terminal as depicted in Figure 5A. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. All experiments were repeated three times. 2 and 648. VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. The mixture was incubate at 37 °C for 30 min, and then the reaction was stopped by the addition of 0. 0%, 0-2. Tris base (100 μL of a 10 mM solution) was added to the wells to solubilize the bound SRB, and absorbance was then read at 515 nm on an automated microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA, USA). To measure the luciferase activity biochemically, cells wereThe activity was measured colorimetrically at 450 nm using a microplate reader (Versamax, Molecular Devices, Sunnyvale, CA, USA). The in vitro differentiation of BMDMs. Microplate Reader Capability > UV - Vis Absorbance. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 4 0112-0126B SafetyInformation InformationaboutthesafeuseoftheinstrumentfromMolecularDevices®includesan Get the most out of SoftMax Pro. After 30-min incubation, the absorbance was measured at 405 nm wavelength using a spectrophotometer device (VersaMax Molecular Devices, Sunnyvale, CA, USA). +44-118-944-8000. The catalytic activity was calculated from the production rate of the hydrolyzed product, p-nitrophenol, by monitoring the absorption (405 nm) at room temperature. S. 1 M sodium phosphate buffer (pH 6. Spectrofluorometer (e. SoftMax® Pro 5 Software from Molecular Devices offers extensive data capture, analysis, and reduction capabilities for its absorbance, luminescence,. $495. Cell viabilities were presented as the ratio of the absorbance of treated cells to the absorbance of control cells cultured with an extract of HDPE. The product formed was then transferred to 96 well plate and the absorbance was measured at 540 nm using microplate reader (VersaMax™, Molecular Devices, CA, USA). Statistical analysisThe absorbance was measured using a microplate reader (VERSAmax; Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 530–560 nm. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. +86-21-6887-8820 fax +86-21-6887-8890 Germany Molecular Devices GmbH Tel. Cell viability was expressed as percent survival of untreated cells. Then the absorbance at 570 nm was measured using a microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA). 1% (w/v) naphtylethylenediamine-HCl], incubated at room temperature for 10 min, and then the absorbance at 540 nm was measured in a microplate reader (VersaMax™, Molecular. Innovative and flexible design features enable accelerated sample preparation time and increased laboratory productivity of DNA, RNA, and protein samples. Absorbance plate reader (e. High-Throughput Screening. Primer design. View the supported Window operating systems for the different versions of the SoftMax Pro software. 9. FAST AND ACCURATE METERTECH Microplate Reader $3,650. Printed in U. That's why our team is at your disposal the majority of time. Phosphodiesterase isozymes: molecular targets for novel antiasthma agents. The calibration curve was fitted to a sigmoid by the four-parameter method in the SoftMax Pro software (Molecular Devices), and allowed the calculation of the concentration of native Antigen 18A. The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis software. The. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Finally, the absorbance at 450 nm was detected by an ELISA reader, VERSAmax (Molecular Devices, San Jose, CA, USA). ~ Bio-Tek ELx808 Microplate Reader $2,795. Model : VersaMax . SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. Add to Cart. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. MOLECULAR DEVICES VersaMax Reader: Model #: VersaMax: Serial #: N/A: Year Built: N/A: Location: California: Price: $3,800. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. Electrolyte leakage analysis was conducted using 8-day-old rice seedlings before and after cold stress treatments in different time points (0, 6, and 11 days) at 4°C. 4 (Molecular Devices cat. The average OD 490 of 12 blank wells (Line 18) was then subtracted from the raw OD 490 obtained for each sample well. Find regional support. HT22 cells are derived from hippocampal neuronal HT4 cells. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Shark chondroitin sulfate A sodium salt (Sigma Aldrich Corp. The cytokine concentration was calculated from the standard curve using seven-parameter curve fitting software (SOFTmaxPro 5. TECHNICAL NOTE. For the MTS assay, the CellTiter 96® AQ ueous One Solution Cell Proliferation Assay kit was used following the manufacturer's instruction. Add to Cart. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 11 0112-0126B Theguidelinesforcuvetteuseintheinstrumentarethesameasthosethatapplytoany high. VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. EC migration was studied by wound healing assay. The activity was measured colorimetrically at 450 nm using a microplate reader (Versamax, Molecular Devices, Sunnyvale, CA, USA). S. 45% of MTT reagent in cell culture media was substituted and incubated for 4 h before adding 100 μL of 100× DMSO. 03 billion doses of. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). g. Filterless. Molecular Devices Spectramax Plate Reader. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. Experimental data analysis was performed with a one-way analysis of variance (Anova with Newman-Keuls post-test) in Prism 6. The system provides automation for up to 50For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The cells were seeded on the bottom of 24-well plates with sterile glass coverslips. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. They were then pre-treated with indicated concentration of drugs for 1 h prior to being treated or not with 5-HT or serum. Accession codes. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. 2. 8), 40 μL of tyrosinase, and 40 μL of L-DOPA were put in each well. Furthermore, VersaMax is reliable, which means more uptime and lower long-term cost of. SpectraMax Plus384 SpectraMax 190 SpectraMax 340PC384 VersaMax Microplate Spectrophotometer Operator s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Quantitative real-time polymerase chain reaction For quantitative real-time polymerase chain reaction (qRT-PCR) analysis, total cellular RNA was extracted from ten zebrafish larvae. Tristar 3 Multimode Microplate Reader. [Google. 00 Regular price. The percentages of inhibition of cell viability were calculated as the ratio between the absorbance of the compound-treated cells and cells with only the vehicle. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. 滤光片型光吸收微孔板读板机（酶标仪），简单易用，性价比高，检测结果精确. Spectranet customer portalThe absorbance was recorded at 532 nm using a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, USA). The absorbance was measured at 570 nm and. Total. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Cell proliferation. The Molecular Devices VersaMax Microplate Reader is a high-performance laboratory instrument designed for fast and accurate measurements of absorbance, fluorescence, and luminescence from 6 to 384-well microplates. Software (optional), such as Softmax Pro (Molecular Devices). The senescence-associated β -galactosidase activity (SA- β -gal) was measured using β -galactosidase Assay Kit following manufacturer's protocol. New and used Molecular Devices VersaMax's for sale on LabX. Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the VERSAmax Tunable Microplate Reader are warranted for a period of one (1) year from the original date of delivery. Then, the absorbance at a wavelength of 450 nm was measured using an absorption microplate reader (VersaMax, Molecular Devices, USA) to evaluate the cell survival rate. to 11. Molecular Devices SoftMax Pro 5. It also integrates with the powerful SoftMax Pro Software for. The VersaMaxTM Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. For pinocytosis, cells in wells were washed in PBS and 5 mg/ml of HPTS dye (in PBS) added and incubated with gentle rocking at 30 °C for 120 min. The plate was read at 570 nm after 5 min using Versamax (molecular devices, United States). 00. Concluding Remarks. ® US HEADQUARTERS. Study conductThe amounts of chlorophyll a and chlorophyll b were measured at 664. ces at one of the following telephone numbers: 408 747-1700 800 635-5577 U. Access all the resources, tools and information you need to stay productive. Then, the cultured cells were treated with different. The absorbance at 570 nm was determined by VersaMax (Molecular Devices, CA) to estimate MTT-formazan production after 24, 48, and 72 hours of incubation to evaluate the proliferation rate. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. In addition, the dissected tissues (heart, liver, spleen, lung and kidneys) were fixed in 4% paraformaldehyde, embedded in paraffin, and sliced into 4-μm thick sections. 3 Methods 1. 2 and 648. Finally, a 5% glucose solution was added to the suspension of CPN and FCPN as a freeze-dried protective agent. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. Assays were conducted in a 96-well microplate using ELISA microplate reader (VersaMax Molecular Devices, USA) and absorbance was measured at 475 nm. This technical note is designed to provide information . Acetylcholinesterase activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction and normalized by protein concentration as determined by a Coomassie. Cell viability was represented by percentage values compared to a control and then used to calculate the number of viable cells. The optical density (OD) was measured at 570 nm (with a reference at 690 nm) by UV-visible spectrophotometer (VersaMax, Molecular Devices, CA, USA) in triplicates. ,, [28]. A. For more information, please visit us at 160 µL of sample solutions/positive control (ascorbic acid) were mixed with 40 µL of DPPH solution and incubated at room temperature for 30 min. 2 mM PLP and 5. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The color was allowed to develop for 20 min in dark and the reaction was stopped with 2. The plate was read at 570 nm using an ELISA reader (VERSAMax, Molecular Devices, VWR, Philadelphia, PA, USA). We would like to show you a description here but the site won’t allow us. Ensure data accuracy of your reader. Select Update Driver. 2. Finally, a 5% glucose solution was added to the suspension of CPN and FCPN as a freeze-dried protective agent. However, whether these cells exhibit the serotonergic neuronal phenotype observed in mature hippocampal neurons. Data were analyzed with GraphPad Prism. Shop on LabX Shop by Category Shop by Brand Shop by Application Shop by Reseller Sell on LabX Sell Your Equipment Advertise with LabX My AccountMolecular Devices Absorbance Microplate Reader VersaMax. Florescence intensity was monitored on a Gemini XPS fluorescence microplate reader (Molecular Devices, Sunnyvale, CA, USA) set for 488 nm excitation and 520 nm emission. We would like to show you a description here but the site won’t allow us. The cellular viability. The blue formazan concentration formed in viable cells was measured at 570 nm using spectrophotometer (Versamax, Molecular Devices, São Paulo, Brazil). The plates were immediately measured at 405 nm for 6 min using a microtiter plate reader (VersaMax; Molecular Devices, Sunnyvale). harveyi BB152 was used as the AI-2-producing positive control (prepared as reported (11)). Statistical Analysis. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. After treatment with GA3The absorbance was measured with a microplate reader (VERSAmax, Molecular devices, San Jose, CA, USA) at 570 nm according to the manufacturer’s instructions. Softmax Pro 소프트웨어가 포함된 PC 워크스테이션 포함. Taking into account the price differences between each seller and each country. 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). A.